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|Authors:||Pawa, Prakash Namdev|
|Title:||ASSESSMENT OF NITRATE REDUCTASE POTENTIAL AND CARBON MONOXIDE SENSITIVITY OF CYTOCHROME c OXIDASE IN THE LEAVES OF CYTOPLASMIC MALE STERILE AND MAINTAINER SEEDLINGS OF PEARL MILLET, Pennisetum glaucum L.|
|Publisher:||MAHATMA PHULE KRISHI VIDYAPEETH, RAHURI - 413 722, DIST AHMEDNAGAR, MAHARASHTRA, INDIA|
|Abstract:||Seedlings of CMS and maintainer lines of pearl millet were grown in soil-filled pots in natural daylight under the green house conditions in the Department of Biochemistry, Mahatma Phule Krishi idyapeeth, Rahuri during the months of March to August 1999 to assess the actual and potential nitrate reductase (NR) activity in the leaves. The laboratory experiments were carried out for the standardization of NR enzyme from the pearl millet system. The differences in the redox state of cytochrome a3 during in situ Ml Abstract contd P.N. Pawar respiration of leaves of CMS and maintainer lines of pearl millet were also examined by an in vivo CO-aerobic assay of NR by sparging CO for one min through leaf segments and by exposing the leaf segments to a defined CO:02 ratio of 5. The in vivo NR assays for leaf materials of 10-day-old pearl millet seedlings were optrndzed in terms of substrate and n-propanol concentrations added into the infiltration media. The optimum concentration of KNO3 added into the infiltration media, required for maximum in vivo NRA, was found to be 100 mM in pearl millet system for the determination of in vivo NRA. When the leaf materials of 10-day-old CMS andmaintainer lines were examined for in vivo NR potential, the enzyme activity was higher in the assays where exogenous nitrate (100 mM) was added (plus KNO3 assays) than that of assays performed without nitrate (minus KNO3 assays). The leaf materials of three CMS and one maintainer lines of pearl millet were examined for carbon monoxidesensitivity of cytochrome c oxidase by sparging CO for one min through weighed leaf fragments (2.5 cm size) and then incubating under aerobic condition for determination of NO2 cumulated in vivo. All the lines were found to be sensitive to CO by this technique. Further, a precisely defined CO:02 ratio of 5 ajpp^^ WI1 Abstract contd P.N. Pawar appropriate for assessing the redox state of cytochrome as in the leaves of CMS and maintainer lines of pearl millet. These studies thus revealed that rather than sparging CO for one min through leaf segments and incubation under aerobic conditions, the technique of exposing leaf segments to a precisely defined CO:C>2 ratio of 5 appears more appropriate in assessing the redox state of cytochrome a3 as reflected in the significant amount of nitrite accumulated in the leaves of pearl millet lines.|
|Appears in Collections:||Thesis|
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