Efficacy of some botanicals and antagonists in the management of damping-off of Chilli (Capsicum annuum L.) caused by Pythium aphanidermatum (Edson) Fitzp
Ahmad, Prof. (Dr.) Shafaat
2018
Ph.D
The experiment was carried out to screen the botanicals and biocontrol agent to study their effect on Pythium aphanidermatum. Also to check the effect of potential botanicals and biocontrol agents on damping-off (Pre and Post-emergence) under pot condition and growth parameter, yield and cost benefit ratio under field conditions.About 60% losses at seedling stage in both nursery and field level have been reported due to damping off disease in chilli caused by Pythium aphanidermatum. It is a soil-borne plant pathogen causing seed rot and damping-off diseases in many vegetables. The efficacy of 23 fungal (Trichoderma spp.), 8 bacterial (Pseudomonas and Bacillus spp.) and 20 leaf extracts were tested for their ability to inhibit the growth of P. aphanidermatum, the causal agent of chilli damping-off. In vitro studies revealed that Trichoderma harzianum, Bacillus subtilis and Lantana camara showed the maximum inhibition of mycelial growth (98.19, 80.03 and 98.88%) followed by Trichoderma asperellum, Pseudomonas fluorescens and Eucalyptus globulus (88.89, 76.28 and 94.08%) of P. aphanidermatum respectively. Effective treatments (5 biocontrol agents viz., T. harzianum, T. asperellum, T. hamatum, B. Subtilis and P. fluorescens and 4 botanicals viz., L. camara, E. globulus, C. roseus and L. inermis) were selected for pot culture evaluation against pre-emergence and post-emergence of chilli. Pooled data of two year of pot trial revealed that the maximum effective treatments were as seed treatment with T. harzianum (2 × 108 CFU ml-1), P. fluorescens (1 × 109 CFU ml-1) and L. camara (20%). These treatments gave maximum protection against pre and post-emergence damping-off and were comparable to the fungicide Ridomil. The possible reasons might have involved in the reduction of damping-off incidence and increased the plant growth and yield of chilli, were antifungal compounds present in biocontrol agents and botanicals were confirmed through GC-MS. Twenty one major compounds were found in ethyl acetate extract of T. harzianum, in which 6 pentyl-α-Pyrone Heneicosane and Harzianic acid are known to have antifungal effect. In methanolic extract of L. camara, β- Caryophyllene, α-Humulene and Caryophyllene oxide were found. Based on above findings, the biocontrol agents and botanicals found safe and effective and may be used as an efficient and eco-safe alternative of chemical fungicide for the management of dampingoff of chilli and for obtaining enhance shoot length, root length, higher yield and cost benefit ratio.
en
160P.
DEPARTMENT OF BIOLOGICAL SCIENCES FACULTY OF SCIENCE SAM HIGGINBOTTOM UNIVERSITY OF AGRICULTURE, TECHNOLOGY & SCIENCES (FORMERLY ALLAHABAD AGRICULTURAL INSTITUTE) ALLAHABAD-211007 UTTAR PRADESH
Efficacy of some botanicals and antagonists in the management of damping-off of Chilli (Capsicum annuum L.) caused by Pythium aphanidermatum (Edson) Fitzp
Botany
Efficacy of some botanicals and antagonists in the management of damping-off of Chilli (Capsicum annuum L.) caused by Pythium aphanidermatum (Edson) Fitzp
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Manisha Pandey Thesis PDF.pdf