STUDIES ON VARIETAL RESPONSE TO ANTHER CULTURE IN RICE (Oryza sativa L.)
Ashok, T. H
2015-07-14
M.Sc
Anther culture is a useful technique in plant breeding to rapidly develop homozygous lines and enhance selection efficiency. This technique applied to rice has been greatly improved and its scope expanded to facilitate other biotechnological approaches. In the present study, ten rice genotypes and different growth regulators were used to know the influence on callus induction and regeneration from the anthers. Among ten genotypes used, Sona Mahsuri and Purple Puttu did not responded to any of the callus induction treatments and highest callus induction was recorded in Jaya (24.81 %) in media containing 2,4-D 2 mg L-1 + Kinetin 1 mg L-1, followed by BI33 (23.43 %) in media containing NAA 2 mg L-1 + Kinetin 1 mg L-1. Furthermore, no callus was induced in media containing no growth regulators and media containing 2,4-D 1mg L-1 + Kinetin 1 mg L-1 callus treatments in any of the genotypes. Among the twenty plantlets regenerated from calli of different genotypes only six were green and the rest were albino. Maximum green plantlet regeneration was from androgenic callus of Jaya genotype obtained from callus induction treatment containing NAA 2 mg L-1 + Kinetin 1 mg L-1 in regeneration treatment containing BAP 1 mg L-1 + Kinetin 1 mg L-1 + NAA 1 mg L-1 (66.66 %). The regeneration treatments containing BAP, Kinetin and IAA produced albino plantlets. Therefore, genotype and types of growth regulator along with its concentration are needed to be considered in order to achieve high callus induction with good regeneration.
en
86
UNIVERSITY OF AGRICULTURAL SCIENCES GKVK, BENGALURU
Plant Biotechnology
STUDIES ON VARIETAL RESPONSE TO ANTHER
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Thesis.pdf
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