Browsing by Author S. B., HARSHITHA,

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2018-08-06Chrysanthemum (Dendranthema grandiflora T), is the second most important flower crop and having different colours grown globally. The introduced cultivar Marigold, which is gaining its popularity in the southern India due to its bright yellow colour and high shelf- life. It is generally propagated through vegetative means, which is a time consuming and having low production rate. Present study was undertaken to standardize the micropropagation and somatic embryogenesis protocol and genetic stability analysis in order to optimize a better substitution for mass multiplication of quality planting material and mitigating the demand of farmers as well as consumers. Nodal segments from the healthy mother plant were sterilized using different surface sterilants and then cultured in MS and B5 media with different growth regulator concentrations for shoot initiation, elongation and rooting followed by hardening in various media. Somatic embryogenesis was undertaken using five different explants and cultured in MS medium with different concentration growth components as well as phytohormones. Among the different media used for micropropagation, B5 media augmented with 1.5 mg/l BAP was found to be best for shoot bud initiation and showed higher rate of multiplication. MS medium with 0.5 mg/l kinetin resulted maximum shoot length whereas B5 medium promoted more number of shoot regeneration. In vitro root initiation was best in MS media and half strength MS media with 1mg/L IBA was next best and was on par with MS media. However, half strength media with 1 mg/L IBA was considered more economical. Among the different explants used for somatic embryogenesis, leaves produced the best quality callus in MS media containing 1 mg/l 2,4-D, 200 mg/l Caesin hydrolysate and 290 mg/l L-Proleine. Shoot tips as explants produced the highest number of shoots per clumps in MS media containing NAA (1.5 mg/l); BAP (3 mg/l); Casein hydrolysate (150 mg/l) and L-Proline (50 mg/l). Ray florets showed the earliest callogenesis as well as plant regeneration in each media. Totally 10 in vitro regenerated plants were randomly selected and tested for genetic stability using two cultivar specific SRAP primers. Tested micropropagated plants were 100 per cent similar with field grown mother plants indicating high efficiency of the optimized micropropagation protocol.KARADIGUDDI, MANJULA; KUKANOOR, LAXMAN; NAIK, RAMACHANDRA; PATIL, CHAYA P.; HADIMANI, H. P.; S. B., HARSHITHA,